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Analyse von Protein-Architekturen und Protein-Ligand-komplexen durch Integrative strukturelle Massenspektrometrie
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal Chemistry
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
DOI:

07:33 min

October 15, 2018

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Chapters

  • 00:04Title
  • 00:45Native MS Acquisition and Analysis for Investigating Protein Complexes and Protein-Ligand Complexes
  • 03:51Acquiring and Analyzing IM-MS
  • 04:34In-Solution Disruption of Protein Complexes for Native MS and IM-MS Led Structure Determination and Investigating Protein Complex Stability using Collision Induced Unfolding
  • 05:45Results: Integrative Structural Mass Spectrometry of Protein Architectures and Protein-Ligand Complexes
  • 07:05Conclusion

Summary

Automatic Translation

Massenspektrometrie (MS) hat als ein wichtiges Instrument für die Untersuchung der Struktur und Dynamik der makromolekularen Versammlungen entstanden. Hier integrieren wir MS-basierte Ansätze, um komplexe Proteinbildung und Liganden Bindung zu befragen.

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