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Samtidige Cryosectioning af flere gnaver hjerner
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Simultaneous Cryosectioning of Multiple Rodent Brains
DOI:

06:37 min

September 18, 2018

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Chapters

  • 00:04Title
  • 00:44Cryoprotection of Perfused Brains and Brain Freezing
  • 03:18Preparing the Megabrain for Sectioning on the Cryostat
  • 04:14Cryosectioning the Megabrain
  • 05:24Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
  • 05:54Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol til at fryse og afsnit hjernevæv fra flere dyr som en tidsbesparende alternativ til forarbejdning enkelt hjerner. Dette reducerer farvning variabilitet under Immunhistokemi og reducerer tid cryosectioning og billedbehandling.

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