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微流控法评价人诱导的多能干细胞内皮细胞 (hipsc-ec) 对白细胞粘附的评价
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
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微流控法评价人诱导的多能干细胞内皮细胞 (hipsc-ec) 对白细胞粘附的评价

DOI:
10.3791/58678-v

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06:47 min

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November 26, 2018

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, , ,
1Department of Anatomy and Embryology,Leiden University Medical Center, 2Department of Applied Stem Cell Technologies,University of Twente

Chapters

  • 00:04Title
  • 00:36Microfluidic Chip Coating and Human Induced Pluripotent Stem Cell-derived Endothelial Cell (hiPSC-EC) Seeding
  • 02:17Live Imaging and Adherent Leukocyte Quantification
  • 05:21Results: Representative Adherent Leukocyte Identification
  • 06:09Conclusion

Summary

Automatic Translation

Automatic Translation

该分步协议提供了实验设置和数据分析的详细描述, 用于评估 hipsc-ec 中的炎症反应和分析生理流下的白细胞粘附。

Tags

MicrofluidicLeukocyte AdhesionHuman Induced Pluripotent Stem Cell-derived Endothelial CellsBiochip CoatingCell SeedingLive ImagingMicrofluidic Pump

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