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Journal / Biochemistry / Kvantifisering av protein samspill nettverk…
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Biochemistry

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JoVE Journal Biochemistry
Kvantifisering av protein samspill nettverk dynamikk ved hjelp av multiplekset co-Immunutfelling
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Journal (Biochemistry)
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
Journal (Biochemistry)
Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
 
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Kvantifisering av protein samspill nettverk dynamikk ved hjelp av multiplekset co-Immunutfelling

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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Kvantitativ multiplex Immunutfelling (QMI) bruker flyt flowcytometri for følsom påvisning av forskjeller i overflod av målrettede protein-protein interaksjoner mellom to prøver. QMI kan utføres ved hjelp av en liten mengde biomaterialet, krever ikke genetisk konstruerte koder, og kan tilpasses for alle tidligere definerte protein interaksjon nettverk.

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Biokjemi IP-FCM Proteomikk protein interaksjon signalering immunutfelling QMI
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