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器官细胞生存在器官细胞切片培养
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Neuroscience
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JoVE Journal Neuroscience
Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures
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器官细胞生存在器官细胞切片培养

DOI:
10.3791/60353-v

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06:31 min

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December 18, 2019

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1Department of Physiology,Northwestern University, Feinberg School of Medicine

Chapters

  • 00:04Title
  • 00:58Organotypic Cerebellar Slice Culture Preparation
  • 01:40Organotypic Cerebellar Slice Culture Dissection
  • 03:32Immunofluorescence
  • 05:24Results: Representative Purkinje Cell Survival in Organotypic Cerebellar Slice Culture Following High Potassium Chloride (KCl) Treatment
  • 05:54Conclusion

Summary

Automatic Translation

Automatic Translation

组织切片培养是研究神经发育或退行/再生过程的有力工具。在这里，我们描述了一个协议，模型在小鼠小脑切片培养中的Purkinje细胞的神经发育死亡。该方法有利于神经保护药物发现的研究。

Tags

Keywords: Purkinje CellOrganotypic Slice CultureCerebellar SlicesNeuroprotectionNeurodegenerative DiseasesCell-cell ConnectionsIn Vivo ConditionsPrimary Cell CultureCerebellumParasagittal Sections

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