A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:58Organotypic Cerebellar Slice Culture Preparation
- 01:40Organotypic Cerebellar Slice Culture Dissection
- 03:32Immunofluorescence
- 05:24Results: Representative Purkinje Cell Survival in Organotypic Cerebellar Slice Culture Following High Potassium Chloride (KCl) Treatment
- 05:54Conclusion
Organotypic slice cultures are a powerful tool to study neurodevelopmental or degenerative/regenerative processes. Here, we describe a protocol that models the neurodevelopmental death of Purkinje cells in mouse cerebellar slice cultures. This method may benefit research in neuroprotective drug discovery.
