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Journal
/
Immunology and Infection
/
在血清型 1
型肺炎链球菌
菌株 519/43 中构建突变体
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Constructing Mutants in Serotype 1
Streptococcus pneumoniae
strain 519/43
Please note that all translations are automatically generated.
Click here for the English version.
在血清型 1
型肺炎链球菌
菌株 519/43 中构建突变体
DOI:
10.3791/61594-v
•
06:06 min
•
September 11, 2020
•
Vanessa S. Terra
,
Charles D. Plumptre
,
Emma C. Wall
,
Jeremy S. Brown
,
Brendan W. Wren
1
Faculty of Infections and Tropical Diseases, Department of Infection Biology
,
London School of Hygiene and Tropical Medicine
,
2
Division of Medicine
,
University College London
,
3
Division of Infection and Immunity
,
University College London UCL
Chapters
00:05
Introduction
00:35
Generation of Plasmid pSD1 and Chemical Transformation of
E. coli
Dh5α
02:03
Plasmid DNA Extraction, Restriction Digestion of pSD1 and Spectinomycin Gene, and Assembly of pSD2
03:03
Transformation of
S. pneumoniae
Strain 519/43
04:46
Results: Confirmation of Mutagenesis
05:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种
肺炎链球菌
血清型1菌株519/43,该菌株可以通过利用其自然获取DNA和自杀质粒的能力进行基因改造。作为原理证明,在肺溶血素(
ply
)基因中制造了同基因突变体。
Tags
Serotype 1 Streptococcus Pneumoniae
Genetic Modification
Plasmid Construction
PSD1
PSD2
Spectinomycin Cassette
Restriction Digestion
Ligation
E. Coli Transformation
S. Pneumoniae Culture
Article
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