A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Biomedical Engineering, Faculty of Engineering,Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering,Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering,Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems,Tel-Aviv University
Chapters
- 00:04Introduction
- 00:50Silicone Strip Preparation
- 01:20Fibrin Gel Polymerization
- 02:29Sample Loading
- 03:42Sample Adequacy Confirmation
- 04:26Stretching Device Operation, Stretching and Imaging
- 05:42Results: Representative Gel Strain Stretching and Gel Fiber Alignment Analysis
- 07:02Conclusion
El método presentado implica el estiramiento uniaxial de hidrogeles blandos 3D incrustados en caucho de silicona mientras permite la microscopía confocal viva. Se demuestra la caracterización de las cepas de hidrogel externas e internas, así como la alineación de la fibra. El dispositivo y el protocolo desarrollados pueden evaluar la respuesta de las células a diversos regímenes de deformación.
