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1Department of Biomedical Engineering, Faculty of Engineering,Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering,Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering,Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems,Tel-Aviv University
Chapters
- 00:04Introduction
- 00:50Silicone Strip Preparation
- 01:20Fibrin Gel Polymerization
- 02:29Sample Loading
- 03:42Sample Adequacy Confirmation
- 04:26Stretching Device Operation, Stretching and Imaging
- 05:42Results: Representative Gel Strain Stretching and Gel Fiber Alignment Analysis
- 07:02Conclusion
La méthode présentée implique l’étirement uniaxial des hydrogels mous 3D incorporés dans le caoutchouc de silicone tout en permettant la microscopie confocale vivante. La caractérisation des souches externes et internes d’hydrogel ainsi que l’alignement des fibres sont démontrés. Le dispositif et le protocole développés peuvent évaluer la réponse des cellules à divers régimes de déformation.
