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Biology
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可视化和量化基于内分泌酶的站点特定DNA损伤
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Biology
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JoVE Journal
Biology
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Please note that all translations are automatically generated.
Click here for the English version.
可视化和量化基于内分泌酶的站点特定DNA损伤
DOI:
10.3791/62175-v
•
10:59 min
•
August 21, 2021
•
Vasiliki Pantazi*
1
,
Ivett Berzsenyi*
1
,
Barbara N. Borsos
,
Tibor Pankotai
1
Institute of Pathology, Faculty of Medicine
,
University of Szeged
Chapters
00:05
Introduction
01:44
Immunodetection of Specific Proteins
05:08
Chromatin Immunoprecipitation
09:06
Representative Results
10:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了免疫染色和染色质免疫沉淀的基本步骤。这些协议通常用于研究DNA损伤相关的细胞过程,并可视化和量化与DNA修复有关的蛋白质的招募。
Tags
DNA Damage
Immunostaining
Chromatin Immunoprecipitation
DNA Repair
Laser Microirradiation
Endonuclease
Double-strand Breaks
Neocarzinostatin
U2OS Cells
Cell Culture
Fixation
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