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Neuroscience
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使用 scRNA-Seq 和 scATAC-Seq 对视网膜基因表达和染色质可及性进行多重分析
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
Please note that all translations are automatically generated.
Click here for the English version.
使用 scRNA-Seq 和 scATAC-Seq 对视网膜基因表达和染色质可及性进行多重分析
DOI:
10.3791/62239-v
•
06:24 min
•
March 12, 2021
•
Kurt Weir*
1
,
Patrick Leavey*
1
,
Clayton Santiago
,
Seth Blackshaw
2,3,4,5,6
1
Solomon H. Snyder Department of Neuroscience
,
Johns Hopkins University School of Medicine
,
2
Department of Psychiatry and Behavioral Science
,
The Johns Hopkins Hospital
,
3
Department of Ophthalmology
,
The Johns Hopkins Hospital
,
4
Department of Neurology
,
The Johns Hopkins Hospital
,
5
Institute for Cell Engineering
,
Johns Hopkins University School of Medicine
,
6
Kavli Neuroscience Discovery Institute
,
Johns Hopkins University School of Medicine
Chapters
00:04
Introduction
00:47
Cell Barcoding
03:23
Paired scRNA-seq and scATAC-seq
04:40
Results: Multiplexed Analysis Using scRNA-Seq and scATAC-Seq for Characterization of Cell Origin, Phenotype and Gene Regulatory Networks
05:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,作者展示了MULTI-seq用于表型的效用,以及随后配对的scRNA-seq和scATAC-seq在表征视网膜转录组和染色质可及性图谱方面的效用。
Tags
ScRNA-seq
ScATAC-seq
MULTI-seq
Gene Regulatory Networks
Gene Expression
Chromatin Accessibility
Development
Disease Progression
Anchor Barcodes
Co-anchor Solution
Cell Preparation
GEM Generation
CDNA Cleanup
Size Selection
Paramagnetic Beads
Isopropanol
Ethanol
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