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Rapid Isolation of Wild Nematodes by Baermann Funnel

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Rapid Isolation of Wild Nematodes by Baermann Funnel

Rapid Isolation of Wild Nematodes by Baermann Funnel

DOI:

10.3791/63287-v

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05:55 min

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January 31, 2022

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Sophia C. Tintori*¹, Solomon A. Sloat*¹, Matthew V. Rockman

¹Department of Biology and Center for Genomics & Systems Biology,New York University

Chapters

00:04Introduction
00:56Collection of the Nematode Substrates
01:43Preparation of an Array of Baermann Funnels
02:21Transferring of Samples into the Funnels
03:23Extraction of Nematodes from the Funnels
04:08Establishing the Cultures
04:45Results: Success of Nematode Isolation by Baermann Funnel
05:18Conclusion

Summary

Automatic Translation

This protocol outlines a method for efficiently extracting live nematodes from natural substrates in the field.

Tags

Keywords: Baermann Funnel Wild Nematodes Caenorhabditis Field Collection Substrate Ecology Population Genetics MetaGenetics Quantitative Genetics Sample Preparation Field Research

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