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JoVE Journal Bioengineering
Somministrazione mediata da elettroporazione di ribonucleoproteine Cas9 e mRNA in epatociti primari di topo appena isolati
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Somministrazione mediata da elettroporazione di ribonucleoproteine Cas9 e mRNA in epatociti primari di topo appena isolati

Article DOI: 10.3791/63828-v • 09:45 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1
1Department of Bioengineering, Clemson University, 2Godley Snell Research Center, Clemson University
* These authors contributed equally

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Questo protocollo descrive le tecniche per isolare gli epatociti primari di topo dal fegato e l'elettroporazione di CRISPR-Cas9 come ribonucleoproteine e mRNA per interrompere un gene bersaglio terapeutico associato a una malattia metabolica ereditaria del fegato. I metodi descritti determinano un'elevata vitalità e alti livelli di modificazione genica dopo l'elettroporazione.

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Bioingegneria Numero 184
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