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Journal / Bioengineering / Elektroporeringsmediert levering av Cas9…
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Bioengineering

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JoVE Journal Bioengineering
Elektroporeringsmediert levering av Cas9 ribonukleoproteiner og mRNA i nyisolerte primære musehepatocytter
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Elektroporeringsmediert levering av Cas9 ribonukleoproteiner og mRNA i nyisolerte primære musehepatocytter

Article DOI: 10.3791/63828-v • 09:45 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1
1Department of Bioengineering, Clemson University, 2Godley Snell Research Center, Clemson University
* These authors contributed equally

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Denne protokollen beskriver teknikker for å isolere primære musehepatocytter fra leveren og elektroporere CRISPR-Cas9 som ribonukleoproteiner og mRNA for å forstyrre et terapeutisk målgen assosiert med en arvelig metabolsk sykdom i leveren. Metodene som er beskrevet resulterer i høy levedyktighet og høye nivåer av genmodifisering etter elektroporering.

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Bioteknologi utgave 184
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