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Biology
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甲基化的DNA免疫沉淀
JoVE Journal
Biology
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JoVE Journal
Biology
Methylated DNA Immunoprecipitation
Please note that all translations are automatically generated.
Click here for the English version.
甲基化的DNA免疫沉淀
DOI:
10.3791/935-v
•
21:24 min
•
January 02, 2009
•
Kelsie L. Thu*
1,2
,
Emily A. Vucic*
1,3
,
Jennifer Y. Kennett
3
,
Cameron Heryet
,
Carolyn J. Brown
,
Wan L. Lam
2,3
,
Ian M. Wilson
3
1
Department of Cancer Genetics and Developmental Biology
,
BC Cancer Research Centre
,
2
Interdisciplinary Oncology Program
,
University of British Columbia – UBC
,
3
Department of Pathology and Laboratory Medicine
,
University of British Columbia – UBC
,
4
Photography/Video Production, Multi-Media Services
,
BC Cancer Agency
,
5
Department of Medical Genetics, Life Sciences Institute,
,
University of British Columbia – UBC
Chapters
00:06
Introduction
02:06
The MeDIP Protocol
02:25
Materials
02:56
DNA Preparation
04:52
DNA Sonication
06:33
Immunoprecipitation of Methylated DNA
13:51
DNA Purification
19:27
PCR Validation
21:09
Credits
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个视频演示的甲基化的DNA免疫(MeDIP)协议。 MeDIP是有选择性地从基因组DNA样本中提取5 - 甲基胞嘧啶甲基化的DNA片段具有特异性抗体(抗- 5 MC)为期两天的过程。
Tags
Methylated DNA Immunoprecipitation
DNA Methylation Patterns
Epigenetics
Gene Expression
Normal Development
Disease
Methylation Profiles
MeDIP
Methylated DNA Extraction
Antibody Reaction
Immunoprecipitated DNA
Input DNA
Heat Denaturation
Anti-5’mC Antibody
Magnetic Beads
Secondary Antibody
Antibody Complex
Separation Of Methylated DNA
Non-methylated DNA Removal
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