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A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
DOI:

12:22 min

April 02, 2012

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Chapters

  • 00:05Title
  • 01:52Isolation and Culture of SCG Neurons from Rat Embryos
  • 05:56Infection of SCG Cultures with HSV-1 Us11-EGFP and Establishment of Latency
  • 08:35Reactivation and Assessment
  • 09:45Results: HSV-1 Reactivation
  • 11:19Conclusion

Summary

Automatic Translation

The protocol describes an efficient and reproducible model system to study herpes simplex virus type 1 (HSV-1) latency and reactivation. The assay employs homogenous sympathetic neuron cultures and allows for the molecular dissection of virus-neuron interactions using a variety of tools including RNA interference and expression of recombinant proteins.

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