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Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
JoVE Journal
Biology
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JoVE Journal Biology
Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
DOI:

09:03 min

January 18, 2018

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Chapters

  • 00:05Title
  • 00:39Fixation and Permeabilization
  • 03:08IFA and RNA-FISH
  • 06:59Results: Viral Transcription Clearly Visible
  • 08:39Conclusion

Summary

Automatic Translation

Viral transcriptional factories are discrete structures that are enriched with cellular RNA polymerase II to increase viral gene transcription during reactivation. Here, a method to locate sites of actively transcribing viral chromatin in 3D nuclear space by a combination of immunofluorescence staining and in situ RNA hybridization is described.

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