A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:39Fixation and Permeabilization
- 03:08IFA and RNA-FISH
- 06:59Results: Viral Transcription Clearly Visible
- 08:39Conclusion
Viral transcriptional factories are discrete structures that are enriched with cellular RNA polymerase II to increase viral gene transcription during reactivation. Here, a method to locate sites of actively transcribing viral chromatin in 3D nuclear space by a combination of immunofluorescence staining and in situ RNA hybridization is described.
