Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

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Summary

We describe a system that utilizes three methods to evaluate the safety and effectiveness of placenta-targeted drug delivery: in vivo imaging to monitor nanoparticle accumulation, high-frequency ultrasound to monitor placental and fetal development, and HPLC to quantify drug delivery to tissue.

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Zhang, B., Chen, Z., Han, J., Li, M., Nayak, N. R., Fan, X. Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods. J. Vis. Exp. (139), e58219, doi:10.3791/58219 (2018).

Abstract

No effective treatments currently exist for placenta-associated pregnancy complications, and developing strategies for the targeted delivery of drugs to the placenta while minimizing fetal and maternal side effects remains challenging. Targeted nanoparticle carriers provide new opportunities to treat placental disorders. We recently demonstrated that a synthetic placental chondroitin sulfate A binding peptide (plCSA-BP) could be used to guide nanoparticles to deliver drugs to the placenta. In this protocol, we describe in detail a system for assessing the efficiency of drug delivery to the placenta by plCSA-BP that employs three separate methods used in combination: in vivo imaging, high-frequency ultrasound (HFUS), and high-performance liquid chromatography (HPLC). Using in vivo imaging, plCSA-BP-guided nanoparticles were visualized in the placentas of live animals, while HFUS and HPLC demonstrated that plCSA-BP-conjugated nanoparticles efficiently and specifically delivered methotrexate to the placenta. Thus, a combination of these methods can be used as an effective tool for the targeted delivery of drugs to the placenta and development of new treatment strategies for several pregnancy complications.

Introduction

Placenta-mediated pregnancy complications, including pre-eclampsia, pregnancy loss, placental abruption and small gestational age (SGA), are common and lead to substantial fetal and maternal morbidity and mortality1,2,3, and very few drugs have been proven to be effective for treating pregnancy disorders4,5. The development of strategies for more selective and safer placenta-targeted drug delivery during pregnancy remains challenging in modern drug therapy.

In recent years, several reports have focused on the targeted delivery of drugs to uteroplacental tissues by coating nanoparticles with peptides or antibodies as placenta-targeted tools. These include an anti-epidermal growth factor receptor (EGFR)6 antibody, tumor-homing peptides (CGKRK and iRGD)7, placenta-targeted peptides8, placental vasculature-targeted peptides9 and antibodies against the oxytocin receptor10.

Here, we demonstrate that a synthetic placental chondroitin sulfate A binding peptide (plCSA-BP) can be used for the targeted delivery of nanoparticles and their drug payloads to the placenta11. The plCSA-BP-guided nanoparticles are complementary to the reported uteroplacental targeting methods because they target the placental trophoblast.

As a non-invasive method, in vivo imaging has been used to monitor placenta-specific gene expression in mice12, and indocyanine green (ICG) has been widely used to track nanoparticles using fluorescence imaging systems13,14,15. Thus, we intravenously injected plCSA-BP-conjugated nanoparticles loaded with ICG (plCSA-INPs) to visualize the plCSA-INP distribution in pregnant mice with a fluorescence imager. We then intravenously injected methotrexate (MTX)-loaded plCSA-NPs into pregnant mice. High-frequency ultrasound (HFUS), another non-invasive, real-time imaging tool16,17 was used to monitor fetal and placental development in the mice. Finally, we used high-performance liquid chromatography (HPLC) to quantify MTX distribution in the placentas and fetuses.

In this protocol, we describe in detail the three-method system used to assess the efficiency of placenta-targeted drug delivery by plCSA-BP-guided nanocarriers.

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Protocol

All mouse experiments strictly followed protocols (SIAT-IRB-160520-YYS-FXJ-A0232) approved by the Animal Care and Use Committee of Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences.

1. Synthesis of Placental Chondroitin Sulfate A-Targeted Lipid-Polymer Nanoparticles

  1. Synthesize MTX- and ICG-loaded lipid-polymer nanoparticles (MNPs and INPs respectively) and plCSA-BP-conjugated nanoparticles (plCSA-MNPs and plCSA-INPs) as described in detail elsewhere18.

2. In vivo Fluorescence Imaging

  1. Preparation of pregnant mice
    1. Place female CD-1 mice (8-12 weeks) with a fertile male of the same strain in one cage (male: female=1:2) in the afternoon and check the vaginal plugs the following morning. If a vaginal plug is observed, define the mouse as embryonic day 0.5 (E0.5).
    2. House pregnant mice alone in a pathogen-free animal room with a 14 h light/10 h dark cycle and provide free access to food and water until E14.5.
  2. Intravenous injection of nanoparticles
    1. Before the procedure, sterilize the nanoparticles by filtration through a 0.22 μm syringe filter. Weigh the pregnant mouse at E11.5 to determine the quantity and volume of nanoparticle injection.
      Note: The nanoparticle injection volume should be less than 1% (volume/weight) of the body weight of the pregnant mouse. For example, the nanoparticle injection volume should be less than 0.25 mL in a 25 g mouse.
    2. To dilate the tail vein, warm the tail for 5-10 min with a heating pad.
    3. Before injection, aspirate the INPs or plCSA-INPs into a 28 g insulin syringe.
    4. Transfer the pregnant mouse to a holding device that restrains the mouse while allowing access to the tail vein. Clean the tail with an alcohol swab. Then insert the syringe into the tail vein. Slowly inject the INPs or plCSA-INPs (5 mg/kg ICG equivalent) with even pressure over 5-10 s.
      Note: Stop injecting if a blister appears on the tail because this outcome indicates that the needle is not in the vein. Syringes should not be shared between mice to minimize disease transmission and cross-contamination.
    5. Record the injection time. Meanwhile, apply gentle pressure to the injection site until the bleeding stops, which normally takes 30-60 s.
  3. In vivo imaging
    1. 30 min after the injection, image the pregnant mice using the in vivo fluorescence imaging system.
    2. Anesthetize the pregnant mice with an oxygen flow rate of 1.0 L/min and isoflurane at 2-4% in an associated chamber of the anesthesia unit and verify full anesthesia by slow and regular breathing. Then, move them into the imaging chamber. Place the anesthetized pregnant mice into the imaging chamber, keeping the animals in a supine position.
    3. Place a nose cone over the mouth and nose to allow the inhalation of 1-2% isoflurane with an oxygen flow rate of 1.0 L/min to maintain anesthesia.
    4. Select 2D-fluorescence and photographic parameters to image the ICG fluorescence signals. Set the exposure to auto and the excitation/emission wavelengths to 710/820 nm.
    5. At the end of the imaging procedure, turn off the isoflurane influx to stop the anesthesia, and carefully return the pregnant mice to their cages.
    6. 48 h after nanoparticle injection, anesthetize the pregnant mice with isofluorane, and then sacrifice the dam by cervical dislocation. Collect the fetuses and placentas using Graefe forceps, Graefe tissue forceps, and dissecting scissors.
    7. Place the placentas and fetuses into the imaging chamber, and image using the method described in step 2.3.4.

3. HFUS Evaluation of Embryonic Development

  1. Animal models
    1. Obtain and prepare the pregnant mice as described in step 2.1.
    2. Use HFUS to image pregnant mice at E 6.5 (Protocols 3.2 and 3.3.3). First, confirm pregnancy by visualizing embryos on day E6.5, and then randomly allocate the pregnant mice into three groups: the MNP group, plCSA-MNP group, and phosphate-buffered saline (PBS) group.
    3. Inject PBS, MNPs or plCSA-MNPs (1 mg/kg MTX equivalent) into the tail veins of the pregnant mice every other day starting at E6.5 as described in step 2.2.
  2. Preparation for imaging
    1. 24 h after the injection of nanoparticles, image the pregnant mice using the HFUS imaging system.
    2. Anesthetize the pregnant mice as described in step 2.3.2. Turn on the integrated temperature controls of the imaging platform and preheat the platform to 37-42 °C. Secure the pregnant mice in a supine position on the platform using tape.
    3. Place the nose cone connected to the anesthesia unit over the snout. Apply 2% isoflurane with an oxygen flow rate of 1.0 L/min to maintain steady anesthesia.
    4. Chemically remove hair from the abdomen using a depilatory cream. Wipe out the residual cream thoroughly with water-soaked gauze, and then coat the abdomen with acoustic coupling gel.
  3. Imaging procedure
    1. Place the 40 MHz transducer in the mechanical arm.
    2. Adjust the transducer position to obtain longitudinal images of the fetus and placenta with the region of interest located in the focal zone.
    3. B-Mode imaging and analysis
      Note: See Movie 1.
      1. Click the B-Mode button and lower the transducer over the abdomen until the fetus and placenta come into view. Press Scan/Freeze to initiate/stop imaging, press Cine store to store the cine loop, and press Frame store to store frame images.
      2. Click the Measure button to analyze the gestational sac length (GS), fetal crown rump length (CRL), biparietal diameter (BPD), abdominal circumference (AC), placental diameter (PD) and placental thickness (PT).
    4. PW-Doppler imaging and analysis
      Note: See Movie 1.
      1. Using the same scan projection, click the PW button, place the sampling volume box in the center of the umbilical artery, and press Scan/Freeze to initiate imaging. Click Cine store to collect umbilical artery images.
      2. Click the Measure button to calculate the umbilical artery peak velocity (UA).
    5. Color Doppler mode imaging and analysis
      1. Using the same scan projection, click the Color button and adjust the transducer position to obtain images of the fetal heart. Press Scan/freeze to initiate imaging and Cine store to collect images.
      2. Click the Measure button to calculate the fetal heart rate (HR).

4. HPLC Analysis

  1. Tissue preparation
    1. Inject the pregnant mice with a single dose of MNPs or plCSA-MNPs (1 mg/kg MTX equivalent) at late pregnancy (e.g., E14.5) as described in step 3.1.3.
    2. After 24 h, anesthetize the mice by an intraperitoneal injection of avertin at 240 μg/body weight (g). Ensure no response to a foot pinch to verify that the mice are fully anesthetized.
    3. Spray the chest area with 75% ethanol. Perform cardiac perfusion (cut the right atria and perfuse through the left ventricle) as previously described in detail19,20 with 50 mL of ice-cold 0.9% saline for 10 min to remove unbound nanoparticles.
    4. Euthanize the dam. Perform a cesarean section to collect the fetuses and placentas using Graefe forceps, dissecting scissors, and Graefe tissue forceps, and store the tissues at -80 °C prior to analysis.
    5. Prepare the homogenization solution (10% perchloric acid) and keep on ice. Collect approximately 200 mg of tissue, and add 500 μL of homogenization solution to each sample. Homogenize the samples using a homogenizer at full speed for 30 s, and repeat this procedure twice.
    6. Centrifuge the samples at 14,000 ×g for 20 min at 4 °C. Filter the supernatant (approximately 300 μL) through a 0.45 μm syringe filter, and transfer the resulting liquid to an HPLC vial. Place sample vials into an autosampler tray for injection.
  2. Preparation of standards
    1. Prepare the following solution for the mobile phase: 40 mM potassium phosphate dibasic (pH 4.5) and acetonitrile (88:12, v/v). Filter the solution through a 0.45 μm pore size syringe filter and transfer the resulting liquid to a clean HPLC reservoir bottle.
      Note: Adjust the pH with 0.1 M phosphoric acid. Use ultrasonic vibration for 15 min to degas the mobile phase each time prior to use.
    2. Weigh 10 mg of MTX into a 1.5 mL centrifuge tube. Add 1 mL of 1 M sodium hydroxide.
    3. Vortex at high speed until the MTX dissolves completely.
      Note: This is the primary stock and can be stored at -20 °C for several months.
    4. To create the secondary MTX stock (500 μg/mL), dilute 50 μL of the primary stock in 950 μL of the mobile phase.
      Note: Store on ice until use, and prepare fresh daily. It is important to use the mobile phase for the preparation of standards to avoid peaks resulting from mixing dissimilar solutions after sample injection.
    5. Make further dilutions to create the standards (Table 1). Store the standards on ice and prepare fresh daily. Run the standards in series with the experimental samples.
Number Final concentration (μg/mL) 500 μg/mL standard, μL Mobile phase(μL)
1 0.5 1 999
2 1 2 998
3 2.5 5 995
4 10 20 980
5 25 50 950
6 50 100 900
7 100 200 800

Table 1. Prepare of standard curve for MTX. The final concentration of MTX standard solution is from 0.5-100 μg/mL.

  1. HPLC instrumentation and operation parameters
    Note: Samples were analyzed on an HPLC system equipped with a solvent pump, a UV spectrophotometric detector (313 nm), and a C18 column (250×4.6 mm, 5 μm particle size).
    1. Turn on the HPLC degasser to remove air from the system. Turn on the flow, equilibrating the column with the mobile phase for 30 min to reduce baseline noise.
    2. Set the temperature of the column to 25 °C, inject 20 μL sample volumes at a flow rate of 1 mL/min, and click the Run Method to start the analysis.
    3. When the runs are complete, manually change the mobile phase to HPLC-grade acetonitrile. Run for approximately 15 min to protect the system.
      Note: Failure to perform this step following the recommended running time could result in damage to the column.
    4. For quantitative analysis, calculate the areas under the standard MTX peaks of interest using the HPLC system software.

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Representative Results

In this manuscript, plCSA-BP-conjugated nanoparticles loaded with MTX (plCSA-MNPs) or ICG (plCSA-INPs) were intravenously injected into pregnant mice. In vivo imaging revealed strong ICG signals in the region of the uterus 30 min after plCSA-INP injection. The INPs were mainly localized to the liver and spleen region (Figure 1A). At 48 h after plCSA-INP injection, pregnant mice were sacrificed, revealing ICG signals only in the placenta, while with no signals were detectable in the fetus (Figure 1B).

We then used HFUS to monitor embryo development after the intravenous injection of nanoparticles. Biometric measurements included the gestational sac length (GS), fetal crown rump length (CRL), biparietal diameter (BPD), abdominal circumference (AC), placental diameter (PD), placental thickness (PT), umbilical artery peak velocity (UA), and fetal heart rate (HR) (Movie 1). The morphological parameters measured at different gestational ages are listed in Table 2. In the plCSA-MNP group, relative to the PBS group, the mean fetal abdominal circumference and umbilical artery peak velocity were significantly decreased at E12.5 (Figures 2A and 2H), and the crown rump length and placental diameter were significantly decreased at E10.5 (Figures 2B and 2F). Beginning on E9.5, the gestational sac length was also significantly decreased (Figure 2C), and the biparietal diameter, placental thickness, and fetal heart rate began to dramatically decrease on E 11.5 relative to those in the PBS group (Figures 2D, 2E and 2G). These findings together suggest that plCSA-MNPs have a strong cytotoxic effect on both fetal and placental development. Interestingly, treatment with MNPs also slightly impaired fetal and placental development (Figures 2A-2H), indicating that nanoparticles might improve the delivery of MTX to the placenta via the enhanced permeability and retention (EPR) effect.

Gestational age Group Decidua (mm)  GS (mm) CRL (mm) BPD (mm) AC (mm) PD (mm) PT (mm) HR (bpm) UA (mm/s)
E6.5 0.92±0.23  /  /  /  /  /  /  /  /
E7.5 PBS / 0.82±0.24 0.72±0.18  /  /  /  /  /  /
MNPs / 0.83±0.14 0.83±0.14  /  /  /  /  /  /
plCSA-MNPs / 0.65±0.23 0.65±0.23  /  /  /  /  /  /
E8.5 PBS / 2.02±0.54 1.88±0.40 0.93±0.23  /  /  /  /  /
MNPs / 1.49±0.50 1.49±0.50 0.82±0.20  /  /  /  /  /
plCSA-MNPs / 1.14±0.46 1.02±0.42 0.83±0.18  /  /  /  /  /
E9.5 PBS / 3.31±0.62 3.49±0.65 1.39±0.54  /  /  /  /  /
MNPs / 2.34±0.68 2.23±0.49 0.98±0.34  /  /  /  /  /
plCSA-MNPs / 1.83±0.42 1.59±0.59 0.94±0.25  /  /  /  /  /
E10.5 PBS / 4.43±0.67 4.97±0.80 2.10±0.61 4.83±1.40 2.91±0.23 2.24±0.24 100±30 30.16±9.40
MNPs / 3.28±0.64 2.91±0.83 1.46±0.54 3.95±1.28 2.66±0.33 2.17±0.19 87±21 24.63±7.35
plCSA-MNPs / 2.64±0.66 2.17±0.85 1.12±0.33 3.82±1.13 2.13±0.35 1.94±0.15 83±22 15.37±5.70
E11.5 PBS / 5.68±0.73 6.45±0.90 3.08±0.70 8.67±2.08 4.16±0.39 2.75±0.26 124±28 31.62±7.76
MNPs / 4.36±0.39 3.74±1.2 2.31±0.53 6.69±1.85 3.56±0.40 2.39±0.23 106±22 25.20±6.18
plCSA-MNPs / 3.42±0.76 2.61±0.84 1.51±0.54 4.59±1.57 2.54±0.49 2.09±0.27 79±20 16.66±5.69
E12.5 PBS / / 8.12±1.29 3.90±0.65 12.43±2.48 5.37±0.42 3.14±0.24 141±26 40.62±10.89
MNPs / / 4.87±1.29 2.87±0.62 8.29±1.78 4.25±0.67 2.65±0.26 119±18 27.76±7.52
plCSA-MNPs / / 3.2±1.28 1.75±0.60 5.47±1.39 3.05±0.50 2.28±0.26 72±22 18.76±7.20
E13.5 PBS / / 10.04±1.2 4.67±0.65 15.64±2.33 6.03±0.60 3.49±0.23 157±28 54.62±12.37
MNPs / / 6.17±1.29 3.37±0.55 9.39±1.88 4.77±0.69 2.92±0.43 109±22 35.84±9.49
plCSA-MNPs / / 3.57±1.71 1.87±0.73 6.25±1.41 3.42±0.63 2.37±0.34 60±23 20.02±11.20
E14.5 PBS / / 12.35±1.6 5.36±0.71 18.38±2.53 6.70±0.64 3.75±0.35 167±27 71.48±10.72
MNPs / / 7.6±1.56 3.90±0.70 10.31±2.31 5.23±0.76 3.10±0.39 99±23 45.80±13.07
plCSA-MNPs / / / / / / / / /

Table 2. Measure morphologic parameters of each gestational age. GS: Gestational sac length; CRL: Crown rump length; BPD: Biparietal diameter; AC: Abdominal circumference ; PD:  Placental diameter; PT: Placental thickness; HR: Fetal heart rate; UA: Umbilical artery peak velocity; /: cannot measure. 

We next measured MTX concentrations in the placentas and fetuses using HPLC. Using the HPLC operation parameters described above, the MTX retention time was determined to be 7 min, and MTX was detected in the placentas of the plCSA-MNP group (Figure 3). The MTX concentrations in placentas and fetuses were determined using MTX standard curves (Figure 4). 24 h after injection, the placental MTX level in the MNP group was significantly lower than that in the plCSA-MNP group, and no MTX was detected in fetuses of the plCSA-MNP group. MTX could still be detected in the placenta 48 h after plCSA-MNP injection (Figure 5). These results demonstrate that plCSA-MNPs cannot cross the placenta, thus minimizing potential adverse effects on the fetus.

In summary, this three-method system comprised of in vivo fluorescence imaging, HFUS, and HPLC can be employed to determine how well a drug delivery vehicle targets nanocarriers and delivers drugs to the placenta. Using these methods, we have demonstrated that plCSA-BP guided nanoparticles are an efficient tool for targeting the delivery of drugs to the placenta.

Figure 1
Figure 1. In vivo fluorescence imaging. (A) Pregnant mice (n=5 each) at E11.5 were injected with INPs or plCSA-INPs (ICG equivalent 5 mg/kg) via the tail vein. After 30 min, the mice were imaged using a fluorescence imaging system. (B) 48 h after the injection of INPs or plCSA-INPs, the fetuses (F, n=2 per mouse) and placentas (P, n=2 per mouse) were collected and imaged with a fluorescence imaging system. Please click here to view a larger version of this figure.

Figure 2
Figure 2. Quantification of embryonic growth by HFUS. (A) The abdominal circumference (n = 30-51 embryos/day), (B) crown rump length (n = 30-51 embryos/day), (C) gestational sac length (n = 10-30 embryos/day), (D) biparietal diameter (n = 30-51 embryos/day), (E) placental thickness (n = 30-51 embryos/day), (F) placental diameter (n = 30-51 embryos/day), (G) fetal heart rate (n = 20-33 embryos/day), and (H) umbilical artery peak velocity (n = 12-36 embryos/day) as measured non-invasively by ultrasound in vivo. All tests were compared by 2-tailed paired t-test, and p < 0.05 was considered statistically significant. Values are expressed as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the PBS group. Please click here to view a larger version of this figure.

Figure 3
Figure 3. Representative HPLC chromatograms of placental samples. Pregnant mice (n=5 each) were intravenously injected with PBS or plCSA-MNPs, and their placentas (n=15 each group) were collected 24 h later for HPLC. Using a standard solution of MTX with UV detection at 313 nm, the retention time was determined to be 7 min. Please click here to view a larger version of this figure.

Figure 4
Figure 4. Standard curves for MTX. The concentrations of MTX ranged from 0.5 μg/mL to 100 μg/mL. The data represent the mean ±SD for n=3. The error bars of some data are smaller than the rhombic symbols. Please click here to view a larger version of this figure.

Figure 5
Figure 5. Application of HPLC to determine the biodistributions of nanoparticles in placentas and fetuses. Pregnant mice were administered a single injection of MNPs or plCSA-MNPs (1 mg/kg MTX equivalent) at gestational stage E13.5. After 24 h and 48 h, the concentrations of MTX in the placentas (n = 15) and fetuses (n = 15) were measured by HPLC. Values are expressed as the means±SD. Differences in MTX concentrations between the MNP and plCSA-MNP groups were analyzed using unpaired Student's t-test (***p < 0.001); nd: not detected. Please click here to view a larger version of this figure.

Movie 1
Movie 1. HFUS images of fetuses and placentas illustrating biometric measurement locations. Please click here to view this video. (Right-click to download.)

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Discussion

In this manuscript, we outline a three-method system for determining whether plCSA-BP-guided nanoparticles are an efficient tool for targeting the delivery of drugs to the placenta. The use of in vivo imaging to monitor the infrared fluorescent ICG signal confirmed the placental targeting specificity of plCSA-BP. Using HFUS and HPLC, we demonstrated that plCSA-BP-conjugated nanoparticles can efficiently deliver MTX only to the placenta cells, not to the fetus.

In the in vivo fluorescence imaging experiments, the gestational ages of pregnant mice are important. The placenta begins to form around E9.521. Additionally, considering the resolution of the imager, the in vivo imaging experiment should be performed after E 10.5. After plCSA-INP injection at E 11.5 according to this protocol, no fluorescence signal was detected with the imager under the described conditions, which may have been due to the skin and internal organs preventing signal transmission22. To overcome this limitation, increasing the injection dose or collection of placentas and fetuses for ex vivo imaging must be utilized.

A critical step in HFUS imaging is the use of a suitable transducer to obtain high-quality embryonic images. The optimized frequency for mouse embryology imaging is 40-50 MHz. Moreover, maintaining the physiological body temperature of the pregnant mouse prior to acquiring images is also important. Finally, the observer should be careful when recording B-mode movies during early embryo development (E 6.5-E 8.5), and this is more dependent on experience. The uncertainty in measurement may be compensated by comparing anatomical features with the frame of reference to fetus and placental movement during ultrasound processing16,23,24. The accuracy of imaging data may be improved by making multiple measurements and increasing the numbers of the fetuses and placentas.

The unbound residual nanoparticle in the blood vessel is an effective factor for evaluating the targeted drug delivery to the placenta and fetus. Thus, cardiac perfusion was performed to remove unbound nanoparticles before the fetuses and placentas were collected. Previous studies7,8,9 have also noted that before analyzing the ability of a peptide to bind the placenta, subjecting the mouse to cardiac perfusion is essential.

A possible pitfall during HPLC analysis is the overlap of MTX with other peaks. Acetonitrile is used to elute MTX from the column. If the overlapping peaks occur before 5 min, decreasing the concentration of acetonitrile in the mobile phase may be helpful. If no peaks or overlapping peaks occur after 30 min, increasing the concentration of acetonitrile is useful. A main limitation of HPLC is that it does not reveal the localization of nanoparticles within the placenta. The plCSA-BP-guided nanoparticles specifically targeted the placental labyrinth in the mouse placenta11. Thus, morphological analysis of the placenta is necessary.

This is the first use of combining in vivo imaging, HFUS, and HPLC to determine the efficiency of placenta-targeted delivery guided by a peptide. HFUS has emerged as an advanced, non-invasive, safe, real-time imaging method and has been used successfully for the high-resolution imaging of mouse embryonic development17,25,26. Although in vivo fluorescence imaging has been widely used to visualize tumor formation and metastasis in live mice27,28,29, it has not previously been used in the study of placental drug delivery. As an alternative approach, in vivo fluorescence imaging has a distinct advantage over HFUS in being able to directly visualize the distribution of intravenously injected nanoparticles in live mice but cannot monitor placental and fetal development. Hence, we combined the advantages of visualization by fluorescence in vivo imaging and high-resolution HFUS-the former enabling visualization of plCSA-BP-guided INPs in vivo, and the latter enabling in vivo monitoring of the effects of plCSA-MNPs on both placental and fetal development and survival. Furthermore, HPLC confirmed that plCSA-MNPs were specifically delivered to placentas and did not reach the fetuses.

Targeted nanomedicine is a new development in the field of pregnancy disorders, and substantial new approaches to specifically deliver drugs to the maternal organs are needed to treat pregnancy disorders in the clinic30. The three-method system described in this protocol is a combination of the in vivo time course imaging of both nanoparticle targeting and the corresponding effects on placental and fetal development, allowing for more precise biochemical measurement of the amount of drug in tissues to evaluate tools for targeted placenta delivery for the treatment of placenta-mediated pregnancy complications.

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Disclosures

X.F. and B.Z. are inventors on patent application PCT/CN2017/108646 submitted by SIAT that covers a placenta-specific drug delivery method and its application. All other authors declare that they have no competing interests.

Acknowledgements

This work was supported by grants from the National Natural Sciences Foundation (81771617) and the Natural Science Foundation of Guangdong Province (2016A030313178) awarded to X.F.; a grant from the Shenzhen Basic Research Fund (JCYJ20170413165233512) awarded to X.F; and the Eunice Kennedy Shriver National Institute of Child Health & Human Development of the National Institutes of Health under Award Number R01HD088549 (the content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health) to N.N.

Materials

Name Company Catalog Number Comments
CD-1 mice Beijing Vital River 201 Female (8-12 week)
Insulin syringe BD 328421 for IV injection
Ethanol absolute Sinopharm Chemical 10009218 for nanoparticles synthesis
Soybean lecithin Avanti Polar Lipids 441601 for nanoparticles synthesis
DSPE-PEG-COOH Avanti Polar Lipids 880125 for nanoparticles synthesis
PLGA Sigma-Aldrich 719897 for nanoparticles synthesis
Ultrasonic processor Sonics VCX130 for nanoparticles synthesis
Methotrexate (MTX) Sigma-Aldrich V900324 for nanoparticles synthesis
Indocyanine green (ICG) Sigma-Aldrich 1340009 for in vivo imaging
phosphate-buffered saline (PBS) Hyclone SH30028.01
IVIS spectrum instrument Perkin Elmer for in vivo imaging
Ultrasound transmission gel Guanggong ZC4252418 for ultrasound imaging
Isoflurane Lunan Pharmaceutical I0040 for maintain the anesthesia
Depilatory cream Nair TMG001 for removing fur
40 MHz transducer VisualSonics MS550S for ultrasound imaging
High-frequency ultrasound imaging system VisualSonics Vevo2100 for ultrasound imaging
Avertin Sigma-Aldrich T48402 for anesthesia
Syringe pump Mindray SK-500III forcardiac perfusion
0.9% saline solution Meilunbio MA0083 forcardiac perfusion
1.5 mL Polypropylene tubes AXYGEN MCT-150-C
-80 °C freezer Thermo Fisher Scientific 88600V
Centriguge Cence H1650R
Perchloric acid Sigma-Aldrich 311421 for precipitating protein
Homogenizer SCIENTZ SCIENTZ-48 for homogenizing tissue
Syringe filter (0.45 μm) Millipore SLHV033RS01
Sodium hydroxide Sinopharm Chemical 10019763 for solving MTX
HPLC vials Waters 670650620 for HPLC
Potassium phosphate dibasic Sinopharm Chemical 20032117 for HPLC
Acetonitrile JKchemical 932537 for HPLC
C18 column Waters 186003966 for HPLC
HPLC system Shimadzu for HPLC

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References

  1. Rodger, M. A., et al. The Association of Factor V Leiden and Prothrombin Gene Mutation and Placenta-Mediated Pregnancy Complications: A Systematic Review and Meta-analysis of Prospective Cohort Studies. PLOS Medicine. 7, (6), e1000292 (2010).
  2. Rodger, M. A., et al. Inherited thrombophilia and pregnancy complications revisited. Obstetrics & Gynecology. 112, (2 Pt 1), 320-324 (2008).
  3. Brenner, B., Aharon, A. Thrombophilia and adverse pregnancy outcome. Clinics in Perinatology. 34, (4), 527-541 (2007).
  4. Fisk, N. M., McKee, M., Atun, R. Relative and absolute addressability of global disease burden in maternal and perinatal health by investment in R&D. Tropical Medicine & International Health. 16, (6), 662-668 (2011).
  5. Fisk, N. M., Atun, R. Market failure and the poverty of new drugs in maternal health. PLOS Medicine. 5, (1), e22 (2008).
  6. Kaitu'u-Lino, T. uJ., et al. Targeted nanoparticle delivery of doxorubicin into placental tissues to treat ectopic pregnancies. Endocrinology. 154, (2), 911-919 (2013).
  7. King, A., et al. Tumor-homing peptides as tools for targeted delivery of payloads to the placenta. Science Advances. 2, (5), e1600349 (2016).
  8. Beards, F., Jones, L. E., Charnock, J., Forbes, K., Harris, L. K. Placental Homing Peptide-microRNA Inhibitor Conjugates for Targeted Enhancement of Intrinsic Placental Growth Signaling. Theranostics. 7, (11), 2940-2955 (2017).
  9. Cureton, N., et al. Selective Targeting of a Novel Vasodilator to the Uterine Vasculature to Treat Impaired Uteroplacental Perfusion in Pregnancy. Theranostics. 7, (15), 3715-3731 (2017).
  10. Paul, J. W., et al. Drug delivery to the human and mouse uterus using immunoliposomes targeted to the oxytocin receptor. American Journal of Obstetrics and Gynecology. 216, (3), e281-e283 (2017).
  11. Zhang, B., et al. Placenta-specific drug delivery by trophoblast-targeted nanoparticles in mice. Theranostics. 8, (10), 2765-2781 (2018).
  12. Fan, X., et al. Noninvasive monitoring of placenta-specific transgene expression by bioluminescence imaging. PloS One. 6, (1), e16348 (2011).
  13. Murata, M., Tahara, K., Takeuchi, H. Real-time in vivo imaging of surface-modified liposomes to evaluate their behavior after pulmonary administration. European Journal of Pharmaceutics and Biopharmaceutics. 86, (1), 115-119 (2014).
  14. Ito, A., et al. New whole-body multimodality imaging of gastric cancer peritoneal metastasis combining fluorescence imaging with ICG-labeled antibody and MRI in mice. Gastric Cancer. 17, (3), 497-507 (2014).
  15. Mazza, M., et al. Liposome-Indocyanine Green Nanoprobes for Optical Labeling and Tracking of Human Mesenchymal Stem Cells Post-Transplantation In Vivo. Advanced Healthcare Materials. 6, (21), (2017).
  16. Greco, A., et al. High frequency ultrasound for in vivo pregnancy diagnosis and staging of placental and fetal development in mice. PloS One. 8, (10), e77205 (2013).
  17. Spurney, C. F., Leatherbury, L., Lo, C. W. High-frequency ultrasound database profiling growth, development, and cardiovascular function in C57BL/6J mouse fetuses. Journal of the American Society of Echocardiography. 17, (8), 893-900 (2004).
  18. Zhang, B., et al. Synthesis and characterization of placental chondroitin sulfate A (plCSA) -targeting lipid-polymer nanoparticles. Journal of Visualized Experiments. (2018).
  19. Devraj, K., Guerit, S., Macas, J., Reiss, Y. An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers. Journal of Visualized Experiments. (132), (2018).
  20. Beeton, C., Chandy, K. G. Isolation of mononuclear cells from the central nervous system of rats with EAE. Journal of Visualized Experiments. (10), 527 (2007).
  21. Watson, E. D., Cross, J. C. Development of structures and transport functions in the mouse placenta. Physiology. 20, (3), 180-193 (2005).
  22. Frangioni, J. V. In vivo near-infrared fluorescence imaging. Current Opinion in Chemical Biology. 7, (5), 626-634 (2003).
  23. Flores, L. E., Hildebrandt, T. B., Kuhl, A. A., Drews, B. Early detection and staging of spontaneous embryo resorption by ultrasound biomicroscopy in murine pregnancy. Reproductive Biology and Endocrinology. 12, 38 (2014).
  24. Khankin, E. V., Hacker, M. R., Zelop, C. M., Karumanchi, S. A., Rana, S. Intravital high-frequency ultrasonography to evaluate cardiovascular and uteroplacental blood flow in mouse pregnancy. Pregnancy Hypertension. 2, (2), 84-92 (2012).
  25. Phoon, C. K. Imaging tools for the developmental biologist: ultrasound biomicroscopy of mouse embryonic development. Pediatric Research. 60, (1), 14-21 (2006).
  26. Pallares, P., Gonzalez-Bulnes, A. Non-invasive ultrasonographic characterization of phenotypic changes during embryo development in non-anesthetized mice of different genotypes. Theriogenology. 70, (1), 44-52 (2008).
  27. Parvani, J. G., Gujrati, M. D., Mack, M. A., Schiemann, W. P., Lu, Z. -R. Silencing β3 integrin by targeted ECO/siRNA nanoparticles inhibits EMT and metastasis of triple-negative breast cancer. Cancer Research. 75, (11), 2316-2325 (2015).
  28. Zhang, B., et al. Targeted delivery of doxorubicin by CSA-binding nanoparticles for choriocarcinoma treatment. Drug Delivery. 25, (1), 461-471 (2018).
  29. Jenkins, D. E., et al. Bioluminescent imaging (BLI) to improve and refine traditional murine models of tumor growth and metastasis. Clinical & Experimental Metastasis. 20, (8), 733-744 (2003).
  30. Keelan, J. A., Leong, J. W., Ho, D., Iyer, K. S. Therapeutic and safety considerations of nanoparticle-mediated drug delivery in pregnancy. Nanomedicine. 10, (14), 2229-2247 (2015).

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