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Journal
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Cancer Research
/
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells
DOI:
10.3791/59629-v
•
07:56 min
•
July 22, 2019
•
Rosalie M. Sterner
2
,
Michelle J. Cox
,
Reona Sakemura
,
Saad S. Kenderian
3
1
Mayo Clinic Medical Scientist Training Program
,
Mayo Clinic College of Medicine and Science
,
2
Department of Immunology
,
Mayo Clinic
,
3
Division of Hematology
,
Mayo Clinic
Chapters
00:04
Title
00:41
T Cell Stimulation and Ex Vivo Culture
02:06
T Cell Transfection and Transduction
03:35
CART19 Cell Expansion
04:48
GM-CSF
k/o
CART19 Production
06:18
Results: Successful Knockout of GM-CSF in CAR-T Cells
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
Here, we present a protocol to genetically edit CAR-T cells via a CRISPR/Cas9 system.
Tags
CRISPR/Cas9
CAR-T Cells
Genetic Modification
T-cell Isolation
T-cell Stimulation
Lentiviral Vector
Lentiviral Production
293T Cells
Transfection
Virus Concentration
Ultracentrifugation
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