Epidermal Laser Wounding: Inducing Localized Damage to Examine Repair Processes in C. elegans
Published: April 30, 2023
Abstract
Source: Xu, S. and Chisholm, A. D. Methods for Skin Wounding and Assays for Wound Responses in C. elegans. J. Vis. Exp. (2014).
This video introduces a method of laser wounding, which is used to cause very precise injuries in the worm epidermis. The sample protocol is from a study of wound response and repair.
Protocol
This protocol is an excerpt from Xu and Chisholm, Methods for Skin Wounding and Assays for Wound Responses in C. elegans, J. Vis. Exp. (2014). 1. Laser Wounding NOTE: Use femtosecond laser irradiation (800 nm) to perform more precise wounding. Prepare agar…
Cite This Article
Epidermal Laser Wounding: Inducing Localized Damage to Examine Repair Processes in C. elegans. J. Vis. Exp. (Pending Publication), e20166, doi: (2023).