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果蝇胚胎的体内离心
JoVE Journal
Biology
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JoVE Journal Biology
In-vivo Centrifugation of Drosophila Embryos
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果蝇胚胎的体内离心

DOI:
10.3791/2005-v

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13:54 min

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June 23, 2010

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,
1Department of Biology,University of Rochester

Chapters

  • 00:00Title
  • 00:43Introduction
  • 01:38Preparing Apple Juice Agar Plates and Harvesting Embryos
  • 03:26Removing the Egg Shell From the Embryos (Dechorionation)
  • 05:09Mounting the Embroys in Agar
  • 08:28Centrifugation and Recovery of Embryos, Followed by Further Processing
  • 13:00Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了独立的细胞器密度的方法，在生活<em>果蝇</em>胚胎。胚胎中嵌入琼脂，离心。这种技术产生沿前后胚胎轴重复性的主要细胞器的分离。这种方法有利于共定位实验和产量的细胞器组分生化分析和移植实验。

Tags

In-vivo CentrifugationDrosophila EmbryosOrganelle SeparationBuoyant DensityIntact EmbryosLiving EmbryosAgar PlateVertical HolesCentrifugationStratificationBright-field MicroscopyFluorescent MarkersColocalizationBiochemical AnalysisTransplantation

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