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Chapters
- 00:00Title
- 00:47Introduction
- 01:01Selecting and Preparing Embryos/Larvae for Live Imaging
- 03:02“Multicolor” in vivo Confocal Imaging
- 05:05Spectral Separation Image Processing Using ImageJ Freeware
- 08:09Representative Images of Neural Loss and Regeneration
- 10:12Conclusion
In deze video worden de technieken voor multicolor confocale time-lapse imaging en gerichte cel ablatie verstrekt. Time-lapse imaging wordt gebruikt om het gedrag van meerdere celtypen van belang te volgen<em> In vivo</em>. Gerichte cel ablatie vergemakkelijkt de studie neurale circuit functie en cel-specifieke neuronale regeneratie paradigma's.
Tags
Multicolor Time-lapse ImagingTransgenic ZebrafishRetinal Stem CellsNeuronal Cell AblationHigh-resolution ImagingLiving Zebrafish LarvaeFluorescent ReportersCellular InteractionsExperimental ManipulationsSerial ImagingFluorophoresHypopigmented Mutant LinesMembrane Targeted Fluorescent ReportersMorphological DetailCellular DetailsChemically-induced AblationNitroreductase Mediated Ablation
