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多色成像定时转基因斑马鱼：可视化由针对性的神经细胞消融激活视网膜干细胞

JoVE Journal
Biology

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JoVE Journal Biology

Multicolor Time-lapse Imaging of Transgenic Zebrafish: Visualizing Retinal Stem Cells Activated by Targeted Neuronal Cell Ablation

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多色成像定时转基因斑马鱼：可视化由针对性的神经细胞消融激活视网膜干细胞

DOI:

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10:31 min

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September 20, 2010

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Junko Ariga*¹, Steven L. Walker*¹, Jeff S. Mumm

¹Department of Cellular Biology and Anatomy,Medical College of Georgia

Chapters

00:00Title
00:47Introduction
01:01Selecting and Preparing Embryos/Larvae for Live Imaging
03:02“Multicolor” in vivo Confocal Imaging
05:05Spectral Separation Image Processing Using ImageJ Freeware
08:09Representative Images of Neural Loss and Regeneration
10:12Conclusion

Summary

Automatic Translation

在这个视频，多色共焦成像时间推移和有针对性的细胞消融技术。定时成像是用来监测多种细胞类型的利益的行为<em>在体内</em>。有针对性的细胞消融有利于研究神经回路的功能和细胞特异性神经再生范例。

Tags

Multicolor Time-lapse Imaging Transgenic Zebrafish Retinal Stem Cells Neuronal Cell Ablation High-resolution Imaging Living Zebrafish Larvae Fluorescent Reporters Cellular Interactions Experimental Manipulations Serial Imaging Fluorophores Hypopigmented Mutant Lines Membrane Targeted Fluorescent Reporters Morphological Detail Cellular Details Chemically-induced Ablation Nitroreductase Mediated Ablation

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