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高通量原位杂交方法表征在胎鼠下泌尿生殖道的基因表达模式
JoVE Journal
Biology
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JoVE Journal Biology
A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract
DOI:

10:24 min

August 19, 2011

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Chapters

  • 00:05Title
  • 01:42Preparing the Urogenital Tissue for Sectioning
  • 02:54Sectioning Urogenital Tissue with a Vibrating Microtome
  • 04:45Sample Basket Preparation
  • 05:34In situ Hybridization
  • 08:46Stained Lower Urogenital Tract Sections
  • 09:45Conclusion

Summary

Automatic Translation

在这里,我们描述了一个高效的高吞吐量<em>原位</em>杂交(ISH)可视化mRNA的表达模式,在胎鼠的前列腺组织切片的方法。该方法可以很容易地适应可视化,在其他的鼠标组织或来自其他物种的组织的mRNA的表达模式。

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