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高效的基因传递到多个中枢神经系统使用新界在子宫内电穿孔
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Neuroscience
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JoVE Journal Neuroscience
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
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高效的基因传递到多个中枢神经系统使用新界在子宫内电穿孔

DOI:
10.3791/2957-v

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13:12 min

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June 23, 2011

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, , , , , ,
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute,University of Calgary , 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute,University of Calgary

Chapters

  • 00:05Title
  • 03:06Set-up and Anesthesia
  • 04:26Animal Preparation for Surgery
  • 05:21Incision and Exposure of Uterine Horns
  • 06:45Injection of DNA into Vesicles
  • 08:23Electroporation
  • 09:13Suturing and Stapling
  • 10:12Fluorescent Protein Expression in Multiple CNS Territories of Electroporated Embryonic Mouse Brain
  • 11:52Conclusion

Summary

Automatic Translation

Automatic Translation

在子宫内电允许在一个空间和时间控制在发展中国家的中枢神经系统（CNS）的方式进行快速的基因传递。在这里，我们描述了一种高适应的在子宫内电的协议，可用于提供成多个胚胎中枢神经系统领域，包括端脑，间脑和视网膜的表达结构。

Tags

Gene DeliveryCNS TerritoriesIn Utero ElectroporationGene ExpressionDevelopmental PathwaysTransgenic TechnologiesLoss-of-function ModelsGain-of-function ModelsMouse TransgenicsAlternative MethodsLive Mouse EmbryosDNA InjectionElectrical CurrentCell MembranesEmbryonic Day (E) 11.5Ultrasound-guided Microinjection ProtocolE15.5 StageParietal Bone DifferentiationFrontal Bone DifferentiationRetina

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