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从三维混合细胞球体共培养的乳腺上皮细胞的隔离
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Mammary Epithelial Cells from Three-dimensional Mixed-cell Spheroid Co-culture
DOI:

07:27 min

April 30, 2012

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Chapters

  • 00:05Title
  • 01:08Establishing Three-dimensional Co-cultures
  • 02:54Isolation of Cells from Spheroid Co-cultures
  • 04:22Representative Imaging and Analysis of the Effects of Fibroblasts on Epithelial Cells Grown in 3D Spheroid Co-cultures
  • 07:05Conclusion

Summary

Automatic Translation

被形容为一个简单的方法,分析相关的上皮细胞组织成纤维细胞的影响。这种方法和三维组织培养相结​​合,可以方便从3D隔离后的细胞分析。该技术适用于不同恶性潜能的细胞,使系统的研究对肿瘤细胞的肿瘤相关基质的影响。

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