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1Department of Radiation Oncology,University of Alabama-Birmingham, 2Department of Radiation Oncology,The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center,University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham
Chapters
- 00:05Title
- 02:03Cell Culture
- 02:51Neutral Comet Assay
- 05:06Alkaline Comet Assay
- 06:09Fixing and Staining Cells
- 06:56Image Acquisition and Analysis
- 07:31Results: Representative Comet Assays Assessing DNA Damage in Hippocampal Neurons
- 09:06Conclusion
Kometen analysen är ett effektivt sätt att upptäcka enkel-och dubbel-strängbrott, inklusive alkali-labila platser och DNA-DNA/DNA-protein tvärbindningar på DNA i alla celler, inklusive hippocampus neuroner. Metoden utnyttjar den differentiella migreringen av DNA i ett elektriskt fält på grund av skillnader i mängden av DNA-skada.
