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1Department of Radiation Oncology,University of Alabama-Birmingham, 2Department of Radiation Oncology,The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center,University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham
Chapters
- 00:05Title
- 02:03Cell Culture
- 02:51Neutral Comet Assay
- 05:06Alkaline Comet Assay
- 06:09Fixing and Staining Cells
- 06:56Image Acquisition and Analysis
- 07:31Results: Representative Comet Assays Assessing DNA Damage in Hippocampal Neurons
- 09:06Conclusion
Det comet assay er en effektiv måde til afsløring af enkelt-og dobbelt-streng pauser, herunder alkali-labile sites og DNA-DNA/DNA-protein tværbindinger på DNA i alle celler, herunder hippocampus neuroner. Fremgangsmåden drager fordel af den differentielle vandring af DNA i et elektrisk felt på grund af forskelle i mængden af DNA-beskadigelse.
