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采用共培养,以检测化学介导的种间相互作用
JoVE Journal
Biology
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Using Coculture to Detect Chemically Mediated Interspecies Interactions
Please note that all translations are automatically generated.
Click here for the English version.
采用共培养,以检测化学介导的种间相互作用
DOI:
10.3791/50863-v
•
08:29 min
•
October 31, 2013
•
Elizabeth Anne Shank
1
Department of Biology
,
University of North Carolina at Chapel Hill
Chapters
00:05
Title
01:33
Prepare and Screen Co-culture Plates
03:20
Isolate Putative Inducing Organisms and Streak for Isolated Single Colonies
04:54
Retest in Secondary Screen to Confirm Hits
05:54
Representative Microbial Interaction Results
07:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细菌产生有可能影响到他们的邻居微生物的生理潜力分泌的化合物。在这里,我们描述了一个共同培养屏幕,可以检测这些化学介导的种间相互作用的混合土壤中的微生物与固体培养基上
枯草芽孢杆菌
的荧光转录记者株。
Tags
Coculture
Interspecies Interactions
Chemically Mediated Interactions
Fluorescent Reporter
Metabolite
Biofilm
Bacillus Subtilis
Environmental Microbes
Microbial Community
Secondary Screen
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