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High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Bredspektret Chemical hæmmere af RNA virus

DOI:
10.3791/51222-v

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11:34 min

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May 05, 2014

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, , , , , ,
1Unité de Génomique Virale et Vaccination, Virology Department,Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department,Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department,Institut Pasteur

Chapters

  • 00:05Title
  • 01:34Preparation of 96-well Daughter Plates with Compounds
  • 03:03Preparation of Cell Cultures to Determine Compound Toxicity
  • 04:18Preparation of Cell Cultures Infected by rMV2/Luc
  • 05:37Luciferase Activity Measurement
  • 06:36Secondary Screening for Toxicity and Broad-spectrum Antiviral Activity
  • 08:52Results: Identification of Anti-viral Hit Compounds
  • 11:06Conclusion

Summary

Automatic Translation

Automatic Translation

In vitro assays til at måle virus replikation er blevet kraftigt forbedret gennem udvikling af rekombinante RNA-virus, der udtrykker luciferase eller andre enzymer, der kan bioluminescens. Her har vi detalje en high-throughput screening rørledning, der kombinerer disse rekombinante stammer af mæslinger og Chikungunya virus at isolere bredspektrede antivirale fra kemiske biblioteker.

Tags

High-throughput ScreeningBroad-spectrum Chemical InhibitorsRNA VirusesCellular PathwaysHost Cell FunctionsAntiviral CompoundsRecombinant VirusesReporter EnzymesBioluminescenceLuciferaseMeasles VirusChikungunya VirusCell Culture Assays

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