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减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默
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Biology
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JoVE Journal Biology
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
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减数分裂纺锤体评估在小鼠卵母细胞的siRNA介导沉默

English

Automatically Generated

11,523 Views

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09:16 min

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October 11, 2015

DOI:

10.3791/53586-v

DOI:
10.3791/53586-v

•

09:16 min
October 11, 2015

•

3 Views
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1Department of Physiology and Pharmacology,College of Veterinary Medicine, University of Georgia

Summary

Automatically generated

在这里，我们提出了一个协议，用于特定的siRNA介导的mRNA枯竭其次是免疫荧光分析来评估减数分裂纺锤体组装和组织在小鼠卵母细胞。这个协议是适合体外转录耗尽的和不同的主轴和/或微管组织中心相关因子中的卵母细胞的功能评价。

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Cite this Article

Baumann, C., Viveiros, M. M. Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing. J. Vis. Exp. (104), e53586, doi:10.3791/53586 (2015).

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