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Isolering av perivaskulær multipotente forløpercelle populasjoner fra humane kardiale Tissue
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1Centre for Cardiovascular Science, Queen’s Medical Research Institute,University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine,University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, 6Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles
Chapters
- 00:05Title
- 01:06Tissue Digestion and Cell Isolation
- 03:27Cell Labelling and Sorting
- 06:05Cell Culture
- 06:54Results: FAS Purification of Myocardial Perivascular Cells and Perivascular Cell Morphology
- 07:39Conclusion
Menneskelig hjertevev havner multipotente perivaskulær forløper cellepopulasjoner som kan være egnet for hjerteinfarkt regenerering. Teknikken som er beskrevet her gjør det mulig for samtidig isolering og rensing av to multipotente stromale cellepopulasjoner forbundet med native blodkar, dvs. CD146 + CD34 - pericytes og CD34 + CD146 - adventitia-celler fra den humane myokardium.
