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Bioengineering
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多显微镜下的血管生成术在基因修饰的 3 d-PLGA/nHAp 支架上进行颅骨临界骨缺损修复
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Visualizing Angiogenesis by Multiphoton Microscopy
In Vivo
in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
Please note that all translations are automatically generated.
Click here for the English version.
多显微镜下的血管生成术在基因修饰的 3 d-PLGA/nHAp 支架上进行颅骨临界骨缺损修复
DOI:
10.3791/55381-v
•
09:34 min
•
September 07, 2017
•
Jian Li
,
Holger Jahr
3
,
Wei Zheng
,
Pei-Gen Ren
1
Center for Translational Medicine Research and Development
,
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
,
2
Department of Orthopedic Surgery
,
Maastricht UMC+
,
3
Department of Orthopaedic Surgery
,
University Hospital RWTH
,
4
Research Laboratory for Biomedical Optics and Molecular Imaging
,
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
Chapters
00:05
Title
00:58
Scaffold Preparation
02:30
Lentivirus Particle Release and Transduction Capacity
03:51
Crtitical Bone Defect Model and Scaffold Transplantation
05:21
In Vivo
Imaging of Angiogenesis within the Bone Defect
07:00
Results: 3D Scaffolds Promote Angiogenesis in Bone Defects
08:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议, 以可视化血管形成
在体内
和在 real-time 的3D 支架的多显微镜。在小鼠颅骨临界骨缺损模型中研究了基因修饰支架的血管生成。治疗组发现更多的新血管。
Tags
Angiogenesis
Multiphoton Microscopy
In Vivo
3D-PLGA/nHAp Scaffold
Calvarial Critical Bone Defect Repair
Blood Vessel Formation
CERES
Extracellular Matrix
Lentiviral Particles
Green Fluorescent Protein
HEK293T Cells
Transduction
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