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Visualisere angiogenese af Multiphoton mikroskopi In Vivo i genetisk modificerede 3D-PLGA/nHAp stillads til Calvarial kritiske knogle defekt reparation
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
DOI:

09:34 min

September 07, 2017

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Chapters

  • 00:05Title
  • 00:58Scaffold Preparation
  • 02:30Lentivirus Particle Release and Transduction Capacity
  • 03:51Crtitical Bone Defect Model and Scaffold Transplantation
  • 05:21In Vivo Imaging of Angiogenesis within the Bone Defect
  • 07:00Results: 3D Scaffolds Promote Angiogenesis in Bone Defects
  • 08:33Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol for at visualisere blodkar dannelse i vivo og i realtid i 3D stilladser af multiphoton mikroskopi. Angiogenese i genetisk modificerede stilladser blev studeret i en murine calvarial kritiske knogle defekt model. Flere nye blodkar blev opdaget i gruppen behandling end kontrol.

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