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Visualisere angiogenese af Multiphoton mikroskopi In Vivo i genetisk modificerede 3D-PLGA/nHAp stillads til Calvarial kritiske knogle defekt reparation
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1Center for Translational Medicine Research and Development,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2Department of Orthopedic Surgery,Maastricht UMC+, 3Department of Orthopaedic Surgery,University Hospital RWTH, 4Research Laboratory for Biomedical Optics and Molecular Imaging,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
Chapters
- 00:05Title
- 00:58Scaffold Preparation
- 02:30Lentivirus Particle Release and Transduction Capacity
- 03:51Crtitical Bone Defect Model and Scaffold Transplantation
- 05:21In Vivo Imaging of Angiogenesis within the Bone Defect
- 07:00Results: 3D Scaffolds Promote Angiogenesis in Bone Defects
- 08:33Conclusion
Vi præsenterer her, en protokol for at visualisere blodkar dannelse i vivo og i realtid i 3D stilladser af multiphoton mikroskopi. Angiogenese i genetisk modificerede stilladser blev studeret i en murine calvarial kritiske knogle defekt model. Flere nye blodkar blev opdaget i gruppen behandling end kontrol.
