A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Bradley Endres; Eug&#233;nie Bass&#232;res; Tasnuva Rashid; Long Chang; M. Jahangir Alam; Kevin W Garey

doi:10.3791/55383

Journal

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Immunology and Infection

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بروتوكول لتوصيف التغيرات المورفولوجية كلوستريديوم ديفيسيل في الاستجابة للعلاج بالمضادات الحيوية

A Protocol to Characterize the Morphological Changes of <em>Clostridium difficile</em> in Response to Antibiotic Treatment

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

بروتوكول لتوصيف التغيرات المورفولوجية كلوستريديوم ديفيسيل في الاستجابة للعلاج بالمضادات الحيوية

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8,927 Views

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12:58 min

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May 25, 2017

DOI:

10.3791/55383-v

DOI:

10.3791/55383-v

•

12:58 min

May 25, 2017

•

5 Views

Bradley Endres¹, Eugénie Bassères¹, Tasnuva Rashid¹, Long Chang², M. Jahangir Alam¹, Kevin W Garey¹

¹Department of Pharmacy Practice and Translational Research,University of Houston College of Pharmacy, ²Department of Electrical and Computer Engineering,University of Houston

Transcript

Summary

Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.