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从头用狂吠/狂暴法生成体细胞干细胞
JoVE Journal
Biology
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JoVE Journal Biology
De Novo Generation of Somatic Stem Cells by YAP/TAZ
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从头用狂吠/狂暴法生成体细胞干细胞

DOI:
10.3791/57462-v

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13:05 min

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May 07, 2018

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, , , , ,
1Department of Molecular Medicine,University of Padua School of Medicine

Chapters

  • 00:04Title
  • 01:07Isolation of Primary Mammary Cell Populations
  • 04:07Purification of Mammary Epithelial Cells by FACS and Seeding of Primary Mammary LD Cells
  • 05:05Induction of YAP-induced Mammary Stem Cells and Sub-culturing of yMaSCs
  • 07:33Generation of yDucts
  • 09:57Results: Reprogramming Primary Mammary LD Epithelial Cells into Mammary Stem Cells
  • 11:25Conclusion

Summary

Automatic Translation

Automatic Translation

在再生医学、疾病建模和了解 SC 特性方面, 可获得的躯体 SCs 是至关重要的。在这里, 我们提出的实验策略, 以重新编程,在体外,分化成人细胞成相应的可膨胀的组织特定的茎/祖细胞由单转录联合激活剂狂吠的瞬态表达。

Tags

Keywords: Somatic Stem CellsYAP/TAZReprogrammingProgenitor CellsDifferentiated CellsRegenerative MedicineCell PlasticityCancerMammary GlandsCell IsolationDissociationCell Viability

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