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JoVE Journal
Immunology and Infection

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小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测
 
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小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测

Article DOI: 10.3791/57463-v 06:52 min May 21st, 2018
May 21st, 2018

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Summary

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我们描述了 NLRP3 inflammasome 激活的细胞基础上的荧光显微镜和染色的主动 caspase-1 和适配器, ASC。以乳酸脱氢酶释放试验为依据, 检测 pyroptotic 裂解的人群。这些技术可以适应研究 inflammasome 生物学的许多方面。

Tags

免疫学和感染 问题 135 免疫学 先天免疫 inflammasome caspase-1 ASC NLRP3 pyroptosis 尼日利亚菌素 小鼠骨髓源性巨噬细胞
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