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3D 染色细胞的包覆和超弹性蛋白样凝胶的制备
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Bioengineering
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JoVE Journal Bioengineering
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
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3D 染色细胞的包覆和超弹性蛋白样凝胶的制备

DOI:
10.3791/57739-v

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11:46 min

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May 19, 2018

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, , ,
1Department of Bioengineering,Stanford University, 2Department of Materials Science and Engineering,Stanford University

Chapters

  • 00:04Title
  • 00:58ELP Expression Protocol
  • 05:06Cell Encapsulation in 3D Elastin-like Protein Hydrogels
  • 07:11Immunocytochemistry of Cells in 3D ELP Hydrogels
  • 09:47Results: Purification of Elastin-like Protein and its Application for 3D Hydrogel Cell Encapsulation
  • 11:18Conclusion

Summary

Automatic Translation

Automatic Translation

重组蛋白工程水凝胶有利于3D 细胞培养, 因为它们允许完整的调谐的聚合物骨干, 因此, 细胞微环境。在这里, 我们描述了重组弹力蛋白样蛋白质纯化的过程及其在3D 水凝胶细胞封装中的应用。

Tags

Elastin-like ProteinHydrogel3D Cell EncapsulationFluorescent LabelingConfocal MicroscopyBiomaterialsRegenerative MedicineCell BiologyCell-extracellular Matrix InteractionsRecombinant Protein ExpressionIPTG InductionCell PelletTEN Buffer

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