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Journal
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Biology
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tchip-seq: 细胞专用表观体分析
JoVE Journal
Biology
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JoVE Journal
Biology
TChIP-Seq: Cell-Type-Specific Epigenome Profiling
Please note that all translations are automatically generated.
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tchip-seq: 细胞专用表观体分析
DOI:
10.3791/58298-v
•
07:28 min
•
January 23, 2019
•
Mari Mito
2
,
Mitsutaka Kadota
,
Shinichi Nakagawa
4
,
Shintaro Iwasaki
5
1
RNA Biology Laboratory
,
RIKEN
,
2
RNA Systems Biochemistry Laboratory
,
RIKEN Cluster for Pioneering Research
,
3
Laboratory for Phyloinformatics
,
RIKEN Center for Biosystems Dynamics Research
,
4
RNA Biology Laboratory, Faculty of Pharmaceutical Sciences
,
Hokkaido University
,
5
Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences
,
University of Tokyo
Chapters
00:04
Title
00:49
Tissue Dissection
01:25
Cell Fixation
04:45
Chromatin Shearing
05:33
Results: Tandem Chromatin Immunoprecipitation Sequencing (tChIP-Seq) Validated
06:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了串联染色质免疫沉淀测序 (tchip-seq) 的分步协议, 该协议可分析细胞类型特异性基因组蛋白的全组蛋白修饰。
Tags
TChIP-Seq
Cell-type-specific
Epigenome Profiling
Chromatin Modifications
Histone Modifications
Neuron-specific
Cryogenic Grinding
Formaldehyde Fixation
Chromatin Isolation
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