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TChIP-Seq: Cell-typspecifika epigenomet profilering
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TChIP-Seq: Cell-Type-Specific Epigenome Profiling
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TChIP-Seq: Cell-typspecifika epigenomet profilering

DOI:
10.3791/58298-v

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07:28 min

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January 23, 2019

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, , ,
1RNA Biology Laboratory,RIKEN, 2RNA Systems Biochemistry Laboratory,RIKEN Cluster for Pioneering Research, 3Laboratory for Phyloinformatics,RIKEN Center for Biosystems Dynamics Research, 4RNA Biology Laboratory, Faculty of Pharmaceutical Sciences,Hokkaido University, 5Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences,University of Tokyo

Chapters

  • 00:04Title
  • 00:49Tissue Dissection
  • 01:25Cell Fixation
  • 04:45Chromatin Shearing
  • 05:33Results: Tandem Chromatin Immunoprecipitation Sequencing (tChIP-Seq) Validated
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver ett stegvisa protokoll för tandem kromatin immunoprecipitation sekvensering (tChIP-Seq) som möjliggör analys av cell-typspecifika genome-wide Histon modifiering.

Tags

TChIP-SeqCell-type-specificEpigenome ProfilingChromatin ModificationsHistone ModificationsNeuron-specificCryogenic GrindingFormaldehyde FixationChromatin Isolation

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