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Journal
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Immunology and Infection
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一种新的生理免疫原树突状细胞生成方法
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种新的生理免疫原树突状细胞生成方法
DOI:
10.3791/59370-v
•
12:08 min
•
May 17, 2019
•
Alessandra Ventura*
1,2
,
Aaron Vassall*
1
,
Alp Yurter
,
Eve Robinson
,
Renata Filler
,
Douglas Hanlon
,
Katrina Meeth
,
Harib Ezaldein
,
Michael Girardi
,
Olga Sobolev
,
Marcus W. Bosenberg
3
,
Richard L. Edelson
1
Department of Dermatology
,
Yale University School of Medicine
,
2
Dermatology Department
,
University of Rome Tor Vergata
,
3
Department of Pathology
,
Yale University School of Medicine
Chapters
00:04
Title
01:37
Collection of Peripheral Blood Mononuclear Cells for Transimmunization
03:09
8-MOP/UVA Treatment of Tumor Cells
04:26
TI Plate Passage of Cells
08:12
Overnight Co-incubation of PBMC with Antigen
09:19
Results: Mouse-to-man Scalable Miniature ECP Device for Cancer Immunotherapy
11:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
开发了转移 (TI) 装置或平板和相关的协议, 以便在实验环境中复制体外光化疗 (ECP) 的主要特征, 从而产生生理激活、可调谐的树突状用于癌症免疫治疗的细胞 (Dc)。
Tags
Dendritic Cells
Physiologic Dendritic Antigen-presenting Cells
PhDC
Monocyte-to-DC Conversion
Platelet Signaling
Anti-tumor T Cell Responses
Monocyte-to-PhDC Maturation
Peripheral Blood Mononuclear Cells
PBMC
Red Blood Cell Lysis
Tumor-bearing Mice
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