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Genetics

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哺乳动物细胞中基于CRISPR的聚集基因屏幕
 
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哺乳动物细胞中基于CRISPR的聚集基因屏幕

Article DOI: 10.3791/59780-v
September 4th, 2019

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Summary September 4th, 2019

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CRISPR-Cas9技术提供了一种有效的方法,可以精确编辑任何细胞类型的哺乳动物基因组,并代表一种执行全基因组基因筛选的新方法。此处提供了一个详细的协议,讨论成功执行池全基因组 CRISPR-Cas9 屏幕所需的步骤。

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