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Caenorhabditis elegans gebruiken om te screenen op weefselspecifieke chaperonne-interacties
JoVE Journal
Biology
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JoVE Journal Biology
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
DOI:

06:55 min

June 07, 2020

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Chapters

  • 00:05Introduction
  • 00:30Non‐Stressful Embryo Synchronization
  • 02:06Common Phenotypic Assays: Animal Cultivation, Embryonic Lethality, and Paralysis
  • 03:30Protein Knockdown Validation
  • 05:02Results: Representative Tissue‐Specific Chaperone Interaction Screening
  • 06:26Conclusion

Summary

Automatic Translation

Om chaperonne-chaperonne- en chaperonne-substraatinteracties te bestuderen, voeren we synthetische interactiescreenings uit in Caenorhabditis elegans met behulp van RNA-interferentie in combinatie met milde mutaties of overexpressie van chaperonnes en monitoren we weefselspecifieke eiwitdisfunctie op organismaal niveau.

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