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Biology
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抗生物質処理された蚊による細菌性経口摂食アッセイ
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A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes
Please note that all translations are automatically generated.
Click here for the English version.
抗生物質処理された蚊による細菌性経口摂食アッセイ
DOI:
10.3791/61341-v
•
09:59 min
•
September 12, 2020
•
Xinyi Liu
2
,
Si Wu
2
,
Wenqian Li
2
,
Meihong Zhang
2
,
Yan Wu
2
,
Ning Zhou
,
Pa Wu
2
1
Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science
,
Hunan Normal University
,
2
State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science
,
Hunan Normal University
,
3
Department of Infectious Diseases, the Second Xiangya Hospital
,
Central South University
Chapters
00:00
Introduction
01:30
Midgut Dissection and Cultivable Bacteria Isolation
04:55
Antibiotic Treatment and Bacterium Reintroduction
09:14
Representative Results
Summary
Automatic Translation
English (Original)
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Automatic Translation
本稿では、蚊中腸の品種微生物の単離と同定、蚊腸内細菌の抗生物質枯渇、特定の細菌種の再導入など、個々の蚊腸内細菌の影響を調査するためのプロトコルを提示する。
Tags
Bacterial Oral Feeding Assay
Antibiotic-treated Mosquitoes
Mosquito Midgut
Gut Microbiota
16S RRNA Gene Sequencing
Antibiotic Treatment
Colony Forming Unit Assay
Mosquito Physiology
Midgut Dissection
Bacterial Isolation
Surface Sterilization
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ネッタイシマカ
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に感染維持
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