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En bakteriel oral fodring assay med antibiotika-behandlede myg
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A Bacterial Oral Feeding Assay with Antibiotic-Treated Mosquitoes

En bakteriel oral fodring assay med antibiotika-behandlede myg

DOI:
10.3791/61341-v

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09:59 min

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September 12, 2020

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, , , , , ,
1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science,Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science,Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital,Central South University

Chapters

  • 00:00Introduction
  • 01:30Midgut Dissection and Cultivable Bacteria Isolation
  • 04:55Antibiotic Treatment and Bacterium Reintroduction
  • 09:14Representative Results

Summary

Automatic Translation

Automatic Translation

Denne artikel præsenterer en protokol til at undersøge effekten af de enkelte myg tarmbakterier, herunder isolering og identifikation af myg midgut kultivable mikrober, antibiotika udtynding af myg tarmbakterier, og genindføre en bestemt bakterie arter.

Tags

Bacterial Oral Feeding AssayAntibiotic-treated MosquitoesMosquito MidgutGut Microbiota16S RRNA Gene SequencingAntibiotic TreatmentColony Forming Unit AssayMosquito PhysiologyMidgut DissectionBacterial IsolationSurface Sterilization

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