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Journal / Immunology and Infection / Digestão de olhos inteiros de rato para análise…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestão de olhos inteiros de rato para análise citométrica de fluxo multi-parameter de fagocitos mononucleares
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Digestão de olhos inteiros de rato para análise citométrica de fluxo multi-parameter de fagocitos mononucleares

Article DOI: 10.3791/61348-v • 09:58 min • June 17th, 2020
June 17th, 2020 •
1, *2, *1
1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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Este protocolo fornece um método para digerir olhos inteiros em uma única suspensão celular com o propósito de análise citométrica de fluxo de vários parâmetros, a fim de identificar populações fagocíticas mononucleares específicas, incluindo monócitos, microglia, macrófagos e células dendríticas.

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Imunologia e Infecção Problema 160 olho citometria de fluxo macrófago microglia monócito mielóide
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