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Demonstratie van heterologe complexen gevormd door Golgi-Resident Type III membraaneiwitten met behulp van Split Luciferase Complementation Assay
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Demonstratie van heterologe complexen gevormd door Golgi-Resident Type III membraaneiwitten met behulp van Split Luciferase Complementation Assay

Article DOI: 10.3791/61669-v • 05:28 min • September 10th, 2020
September 10th, 2020 •
1, 1, 1
1Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw

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Het hier gepresenteerde protocol is bedoeld om het optreden van heterologe interacties tussen Golgi-residente type III membraaneiwitten met cytoplasmatisch blootgestelde N- en/of C-termini in levende zoogdiercellen aan te tonen met behulp van de meest recente variant van de split luciferase complementatie assay.

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Biologie Nummer 163 split luciferase complementatie assay eiwit-eiwit interacties PPI's luminescentie bioluminescentie heterologe complexen Golgi apparaat type III membraaneiwitten nucleotide suikertransporters NST's voorbijgaande transfectie
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