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使用分裂荧光素酶互补测定法展示高尔基体驻留III型膜蛋白形成的异源复合物
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Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
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使用分裂荧光素酶互补测定法展示高尔基体驻留III型膜蛋白形成的异源复合物

English

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2,196 Views

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05:28 min

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September 10, 2020

DOI:

10.3791/61669-v

DOI:
10.3791/61669-v

•

05:28 min
September 10, 2020

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3 Views
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1Laboratory of Biochemistry, Faculty of Biotechnology,University of Wroclaw

Summary

Automatically generated

这里提出的方案旨在使用分裂荧光素酶互补测定的最新变体，证明高尔基体驻留的III型膜蛋白与活哺乳动物细胞中细胞质暴露的N-和/或C-末端之间异源相互作用的发生。

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Cite this Article

Wiertelak, W., Olczak, M., Maszczak-Seneczko, D. Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay. J. Vis. Exp. (163), e61669, doi:10.3791/61669 (2020).

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