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1Department of Biomedical Engineering, Faculty of Engineering,Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering,Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering,Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems,Tel-Aviv University
Chapters
- 00:04Introduction
- 00:50Silicone Strip Preparation
- 01:20Fibrin Gel Polymerization
- 02:29Sample Loading
- 03:42Sample Adequacy Confirmation
- 04:26Stretching Device Operation, Stretching and Imaging
- 05:42Results: Representative Gel Strain Stretching and Gel Fiber Alignment Analysis
- 07:02Conclusion
Den præsenterede metode indebærer uniaxial strækning af 3D bløde hydrogels indlejret i silikone gummi og samtidig tillade levende confocal mikroskopi. Karakterisering af de eksterne og interne hydrogelstammer samt fiberjustering demonstreres. Den udviklede enhed og protokol kan vurdere cellernes reaktion på forskellige stammeregimer.
