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Polarização de Células e Análises Derivadas de Monócitos Humanos M1 e M2 com Citometria de Fluxo sobre Infecção por Mycobacterium tuberculosis
This content is Free Access.
1Center for Infectious Medicine (CIM), Department of Medicine Huddinge, ANA Futura,Karolinska Institutet, 2Infectious Diseases Division,International Centre for Diarrhoeal Disease Research, Bangladesh, 3Clinical Microbiology, Department of Laboratory Medicine (Labmed), ANA Futura,Karolinska Institutet, 4Childhood Cancer Research Unit, Department of Women’s and Children’s Health,Karolinska Institutet
Chapters
- 00:04Introduction
- 00:44Peripheral Blood Mononuclear Cell (PBMC) Isolation
- 01:40Monocyte-Derived Cell Differentiation and Polarization
- 03:25Mycobacterium tuberculosis (Mtb) Culture
- 05:00Monocyte-Derived Cell Mtb Infection
- 06:03Flow Cytometry Staining and Analysis
- 07:40Results: Representative Phenotypic M1 and M2 Cell Characterization Before and After Mtb Infection
- 09:47Conclusion
Este protocolo fornece um método para estudar a infecção por Mycobacterium tuberculosis em macrófagos m1 ou M2 polarizados humanos baseados na diferenciação de monócitos periféricos-sanguíneos para células semelhantes a macrófagos que são infectados com a cepa virulenta H37Rv, e analisado com citometria de fluxo usando um painel de 10 cores, incluindo a expressão de marcadores M1/M2 selecionados.
